Journal: The Journal of Biological Chemistry

Article Title: An enhancement of extrachromosomal circular DNA enrichment and amplification to address the extremely low overlap between replicates

doi: 10.1016/j.jbc.2026.111302

Figure Lengend Snippet: Comparison of extrachromosomal circular DNA (eccDNA) characteristics between OPM-2 myeloma cells cultured and xenografted . A , graphic depicting 7-week-old immunodeficient NSG mice injected intravenously with 10 5 OPM-2 MM cells. B , bar graphs showing mean eccDNA/Mb, mean concatemeric read percentage, and mean concatemer-derived eccDNA of in vitro cultured OPM-2 cells ( n = 3). C , violin plot showing the length of eccDNA in vitro cultured OPM-2 cells. D , half-moon chart showing percent eccDNA carrying features of in vitro cultured OPM-2 cells ( n = 3). E , bar plot showing percent overlap of eccNDA between two and three replicates of in vitro cultured OPM-2 cells. F , bar graphs showing mean eccDNA/Mb, mean concatemeric read percentage, and mean concatemer-derived eccDNA of OPM-2 xenograft ( n = 7). G , violin plot showing the length of eccDNA in OPM-2 xenografts. H , half-moon chart showing percent eccDNA carrying features of OPM-2 xenografted ( n = 7). I , bar plot showing percent overlap of eccDNA across all permutations of OPM-2 xenografted ( n = 7). Examples of overlapped eccDNAs identified from cultured ( J ) OPM-2 cells, ( K ) xenografted OPM-2 cells, and ( L ) all of them. See lanes annotation in supplementary information . The bars show mean ± standard deviation (SD) of biological replicates. Boxes show median and interquartile range (IQR).

Article Snippet: For the xenografted animal studies, 7-week-old immunodeficient NSG (RRID: IMSR_JAX:005,557) mice were injected intravenously with 10 5 OPM-2 multiple myeloma cells and sacrificed after 3 weeks, as previously reported ( ).

Techniques: Comparison, Cell Culture, Injection, Derivative Assay, In Vitro, Standard Deviation